WebApr 9, 2024 · The membranes were blocked with the Quickblock fast blocking solution (Beyotime Biotechnology) for 15–20 min and incubated with Klotho (Product # PA5-88303), FoxO1 (ab52857),Phospho-FoxO1 (Ser256) (CST #9461), IL-6 (ab 6672, Abcam), γH2AX (ab 11174), PARP-1 (CST #9532), p16INK4a (ab 81278), peroxisome proliferator … WebThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. Concentration. 0.5 mg/mL. Storage & Handling. The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Anti-Histone H2A.X antibody (ab11175) Abcam
WebBonner’s team, collaborating with CCR colleague Andre Nussenzweig, Ph.D., established that H2AX is an acute sensor of a particularly harmful type of DNA damage called a double-strand break. When such damage … WebSpecificity. ab124781 does not work in ICC/IF on PFA fixed cells but does work on methanol fixed cells. The immunogen used for this product shares full homology with other H2A proteins, including H2A1, H2A1B, H2A2A and H2AZ. Cross-reactivity with these proteins has not been confirmed experimentally. high cpu idle temps
Platinum-Induced Ubiquitination of Phosphorylated H2AX by …
WebApr 30, 2024 · For western blot of endogenous proteins, anti-gH2AX (Cell Signaling Technology (CST), MA #9718, 1:1000), anti-Actin B (CST, MA #4970, 1:1000), anti-p … WebThe phosphorylated form of this Ab is known as gamma H2A.X, when phosphorylated at Ser 139. The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. WebFeb 4, 2010 · Abstract. Phosphorylation of the Ser-139 residue of the histone variant H2AX, forming γH2AX, is an early cellular response to the induction of DNA double-strand … high cpu svchost netsvcs