site stats

Phospho 4ebp1 antibody

WebNov 11, 2008 · To determine whether CDKs could be responsible for the rapamycin-stimulated rephosphorylation of 4EBP1, we treated cells with saturating concentrations of roscovitine (50 μM), a CDK inhibitor. ... Phospho-S6 and actin were blotted for normalization. (D) The experiments were completed in the same way as in C, except WT 4E-BP1, AA 4E … WebInvitrogen Anti-Phospho-4EBP1 (Thr37, Thr46) Polyclonal, Catalog # PA5-114544. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Immunohistochemistry (PFA fixed) (IHC (PFA)) applications. This antibody reacts with Human, Mouse, Rat samples. Supplied as 100 µL purified antibody (1 mg/mL).

磷酸化4E结合蛋白1抗体_价格-品牌-详情介绍_丁香通

Web(orb502277) 4EBP1 (phospho-Ser65/Thr70) antibody (Biotin) - Biorbyt - CiteAb A Biotin-conjugated rabbit polyclonal antibody, raised against Eukaryotic translation initiation factor 4E-binding protein 1 (Mouse), supplied by Biorbyt. WebJun 25, 2010 · Anti-phospho Drosophila S6K, anti-S6K, anti-phospho S6K, anti-Akt, anti-phospho Akt, and anti-phospho 4EBP1 antibodies were from Cell Signaling. Anti-Myc, anti-HA, and anti-FLAG antibodies were from Santa Cruz Biotechnology, Covance, and Sigma, respectively. ... stimulation for 30 min. Phosphorylation and protein levels of dS6K were … simon reward card american express https://mintpinkpenguin.com

Phosphorylated 4EBP1 is associated with tumor progression and …

WebThis antibody detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1. Tested applications Suitable for: IHC-P, WB more details Species reactivity Reacts with: Rat, Human Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan Immunogen WebView Rabbit Polyclonal anti-4EBP1 [p Thr46, p Thr37] Antibody (NBP3-13169). Validated Applications: WB. Validated Species: Human, Mouse, Rat + more. Validated by: Biological Strategies, Genetic Strategies. WebAll lanes : Anti-eIF4EBP1 (phospho S65) antibody [EPR23889-3] at 1/1000 dilution Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate Lane 2 : HeLa treated with 100nM rapamycin for 24 hours, whole cell whole cell lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at … simon r green haunted by the past

Phospho-4E-BP1 (Thr70) Antibody from Cell Signaling Technology

Category:Purified anti-4EBP1 Phospho (Thr70) Antibody - BioLegend

Tags:Phospho 4ebp1 antibody

Phospho 4ebp1 antibody

4EBP1 [p Thr46, p Thr37] Antibody (NBP3-13169): Novus Biologicals

WebFeb 10, 2024 · Cell Signaling Technology's Phospho-4E-BP1 (Thr70) Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Precipitation, Immunoprecipitation, and Western Blot. Phospho-4E-BP1 (Thr70) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 70. Web实验用到的供体是Thr46(铽标记的anti-phospho-4EBP1),受体是绿色荧光标记的4EBP1,是mTOR下游信号通路的蛋白,有活性的mTOR能磷酸化4EBP1,使之与供体结合。如果mTOR活性受到抑制,供体受体距离不能达到荧光能量转移的要求,则在515nm处与485nm处的信号比值增大。

Phospho 4ebp1 antibody

Did you know?

WebAnti p-eIF4EBP1 (Ser65/Thr70) antibody, Anti BP1 antibody, Anti BP 1 antibody, Anti eIF4EBP1 (phospho Ser65/Thr70) antibody, Anti PHAS I antibody, Anti 4EBP1 antibody, Anti PHASI antibody, Anti 4E-BP1 antibody, Anti eIF4E-binding protein 1 antibody, Anti eIF4EBP1 (phospho S65/Th70) antibody, Anti 4E BP1 antibody, Anti eIF4E binding protein 1 … WebProduct Description. Rabbit anti 4EBP1 antibody recognizes an epitope within the C-terminal region (CT) of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1), a rapamycin-sensitive downstream target of TOR (Target of rapamycin), and regulatory factor of eukaryotic mRNA translation. The association between 4EPB1 and eukaryotic ...

WebMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser65 of mouse 4E-BP1. Background Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Webp-4E-BP1 Antibody (62.Ser 65): sc-293124. Western blot analysis of 4E-BP1 phosphorylation in HeLa (A), K-562 (B) and HEL 92.1.7 (C) whole cell lysates. Description Gene Info Datasheets and Protocols The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5′ end of mRNA.

WebPhospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb WebAbcam - antibodies and reagents supplier, find any antibody Hello. We're improving abcam.com and we'd welcome your feedback. ... 4ebp1 35 results for 4ebp1 ... Anti-eIF4EBP1 (phospho S65) antibody [EPR23889-3] - BSA and Azide free (ab283288)

WebPhospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence.

WebPhospho-4E-BP1 (Ser65) Antibody detects endogenous levels of 4E-BP1 when phosphorylated at serine 65. This antibody has been shown to also recognize 4E-BP1 when phosphorylated at serine 101 (See application reference, Wang et al. 2003). Species … simon reynolds wetherbysimon r go chiropractic incWeb4EBP1 is a 118 amino acid protein with a predicted molecular weight of 13 kD. Distribution Cytosol and nucleus/ubiquitously expressed Function Regulation of global translation Biology Area Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction Molecular Family Phospho-Proteins Antigen References simon rhodes st johns chambersWebRabbit anti 4EBP1 (pSer65) antibody recognizes eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1) also known as phosphorylated heat-and acid-stable protein regulated by insulin 1 (PHAS-I), when phosphorylated at serine 65. 4EBP1 belongs to the eIF4E-binding protein family. simon reuben orthopaedic surgeonWebProduct name Anti-eIF4EBP1 (phospho T70) antibody [EPR654 (2)A] See all eIF4EBP1 primary antibodies Description Rabbit monoclonal [EPR654 (2)A] to eIF4EBP1 (phospho T70) Host species Rabbit Tested applications … simon rhee autographWebThe lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -2℃ . When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. 溶质携带物家族-1抗体, ... simon rhodes ministry of defenceWebEach 4EBP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our 4EBP1 Antibodies can be used in a variety of model species: Human, Mouse, Porcine, Rat. simon r green hawk and fisher